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Learn How To Harness The Power of PCR!
What do the following activities have in common:
Cancer research
Identification of plant viruses
Portable microchip DNA profiling
Detection of viruses in blood donations (including West Nile Virus)
HIV/AIDS research?
The answer-every one of them can be done quickly, effectively and more accurately than ever using the Polymerase Chain Reaction (PCR) technique! PCR makes it possible to produce literally billions of copies of a specific stretch of DNA in just a few hours, even if you only have a tiny sample. PCR is easy to perform, highly sensitive and extremely effective at avoiding cross-contamination of samples – a combination of features that makes this exciting technology useful for a wide variety of applications in molecular diagnostics and analysis.
PCR has become faster and easier than ever. For example, crime scene investigators can now carry portable microchip DNA profiling devices in the field and complete a PCR analysis of a DNA sample in just minutes. PCR has also made it possible to efficiently analyze mitochondrial DNA (mtDNA) in a faster more accurate manner.
This powerful technique is now used by professionals in medicine, biotechnology, law enforcement, environmental protection, food science and other disciplines where fast and reliable DNA generation, characterization and analysis is needed. If you want to enhance your career in any of the fields that use the power of PCR, this two-day hands-on course is exactly what you need.
Over 400 Of Your Colleagues Can’t Be Wrong!!
Our popular course has been updated and revised annually as PCR technology has evolved! Over 400 professionals from companies including Bristol-Myers Squibb., Roche Diagnostic Systems, Schering-Plough, Johnson & Johnson, FMC Corporation, Glaxo Smith Kline, and Sanofi Aventis have attended our practical course.
Program Description:
During this comprehensive program, you will learn how to conduct the PCR reaction through hands-on laboratory exercises using state-of-the-art equipment. In addition, three lectures will cover PCR’s fundamental concepts and the most popular applications, including:
DNA fingerprinting/forensics
Genome mapping, phylogenetics
Diagnosis of genetic disease/disease susceptibility
Diagnosis of pathogens
Quantitative real-time PCR
Isothermal amplification
Whole genome amplification.
Specific topics by session:
Laboratory Session
PCR techniques such as amplification of genomic DNA
Amplification of RNA template
Random amplified polymorphic DNA (RAPD) analysis
Basics of PCR
Lecture Session:
Basics of PCR (PCR reaction and necessary controls; maximizing the PCR yield; analysis of PCR products; accuracy of PCR; cloning and sequencing of PCR products; real time PCR—Taqman and other assays)
Variations of standard PCR (RNA PCR; nested, inverse and asymmetric PCR; use of degenerate primers; multiplex and long-range PCR; mutagenesis of target DNA; RAPD analysis; isothermal applications; whole genome amplification).
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